I was updating a site from MOSS 2007 -> MSS 2010 and I tried to access the site and kept getting a blank screen and the word "Done" in the bottom left hand corner of the screen. So I looked in the IIS log and noticed that there were a lot of 401.1 and 401.2 entries in it.
So immediately thought of the LoopBack Test that used to happen in MOSS 2007 and sure enough after I added the appropriate Registry Key it all burst back into life !
Just because it is MSS 2010 it does not mean that the old MOSS 2007 gotchas have gone away.